A Level Biology Revision Lesson 359: - Electrophoresis
Task 1. Check Where this Lesson fits into your Exam Specification!
Task 2. Watch the Revision Notes Lesson in Full.
00:00 Learning Outcomes
00:22 What is Gel Electrophoresis?
01:00 How does Gel Electrophoresis work?
02:50 Small DNA fragments migrate further an quicker...
03:15 Remember DNA is mixed with a loading dye...
03:49 Watch VNTRs (Genetic fingerprinting next)
04:14 Exam style Q and A (1).
04:40 Exam style Q and A (2).
Check Your Spec!
★ AQA A Level Biology Specification Reference: - 126.96.36.199 Genetic fingerprinting (A-level only). Students should be able to: interpret data showing the results of gel electrophoresis to separate DNA fragments.
★ CIE A Level Biology Specification Reference: - 19.1 Principles of genetic Technology: d) describe and explain how gel electrophoresis is used to analyse proteins and nucleic acids, and to distinguish between the alleles of a gene (limited to the separation of polypeptides and the separation of DNA fragments cut with restriction endonucleases)
★ Edexcel A Level Biology (Biology A – Salters-Nuffield) Specification Reference: - Topic 6: Immunity, Infection and Forensics. Use gel electrophoresis to separate DNA fragments of different length.
★ Edexcel A Level Biology (Biology B) Specification Reference: - N/A. Note; Electrophoresis is NOT specifically mentioned in the spec. but understanding it is essential for Topic 7: Modern Genetics. 7.1 Using gene sequencing - in particular in forensic science, to identify criminals and to test paternity, using DNA profiling.
★ OCR A Level Biology (Biology A) Specification Reference: - 6.1.3 Manipulating genomes (e) the principles and uses of electrophoresis for separating nucleic acid fragments or proteins
★ OCR A Level Biology (Biology B) Specification Reference: - 5.1.3 Gene technologies (d) the principles and uses of agarose gel electrophoresis.
★ WJEC A Level Biology Specification Reference: - 7. Application of reproduction and genetics: (d) the use of PCR and electrophoresis to produce a genetic fingerprint; the forensic use of genetic fingerprinting.